Scientific Insights

Explore the role of parental cell lines including HEK293T, CHO-K1, Daudi, Hela, and more. View information about specific cell line variants.

Dive into the essential role of cyclic AMP in cellular signaling and its impact on drug discovery research. Understand how this key messenger influences biological processes and therapeutic development, offering valuable insights for researchers in the field.

Explore Multispan’s expert tips for establishing successful cell-based assays in the third part of our blog post series.

Explore Multispan’s expert tips for establishing successful cell-based assays in the second part of our blog post series.

Explore Multispan’s expert tips for establishing successful cell-based assays in the first part of our blog post series.

GTPγS assay is an integral part of the MULTISCREEN GPCR assay platform, utilizing high-quality HTS-ready membrane and cell lines to provide answers to questions.

MULTISCREEN Receptor Internalization Assay permits high throughput screening of GPCR receptor activity, independent of downstream signaling. It allows screening regardless of ligand or key signaling modalities maybe unknown. It also provides insights into receptor pharmacology to help triage drug lead candidates.

Multispan brings its expertise in cell-based assay development to the functional analysis and validation of targets for precision medicine. We discuss applications in rare disease and pharmacogenomics of key target classes

In this paper, we discuss the characteristics of GPCRs that make it so difficult to develop effective anti-GPCR antibodies. We then discuss Multispan’s customized MULTISCREEN GPCR FACS binding and functional assays for antibody profiling.

Multispan’s panel of GPCR orthologs can enable critical assessment of species-specific variations in target binding activity and modulation of GPCR signaling that could impact in vivo efficacy in small and large animal models.

GPCRs have historically been among the most important classes of pharmaceutical targets across a wide range of clinical indications. Recent clinical, scientific and technical advances have also opened new therapeutic avenues to exploit members of this class. In this paper, we discuss how Multispan’s MULTISCREEN GPCR panel can provide important insights during the development of new compounds and in repurposing existing therapies.

How mutagenesis enables understanding structure/function relationships of GPCRs. Read full article.

With over 16 years of in vitro safety pharmacology experience, Multispan leads the way in providing researchers with the tools needed… Read full article.

Assay Orphan GPCRs in High Throughput with MultiScreen Beta-Arrestin Sensor Technology. Read and download full article.

Hit-to-Lead and Lead Optimization In Vitro Pharmacology Support. Saving time, energy and cost from target-to-IND. Read and download full article.

2020 was a banner year for CXCR7. Two research studies and several great review articles were published that delve into the biased nature of CXCR7 signaling, its role in cancer, and the development of CXCR7-selective inhibitors.

CRISPR Breaks into GPCR Research Again Delineating the Role of GRK2/3 in Opioid Receptor Internalization. Read full scientific insight. View all scientific insights.

The foundational role of relevant, robust cell-based assays in covid antiviral and vaccine development.

Harness the power of freeing your GPCR from unwanted tags using Multispan’s proprietary MultiScreen™ β-Arrestin sensor technology.

The Power of Integrating Screening and Assay Development.

Explore the many types of ligand binding assays using GPCR membranes and the importance of high-quality membranes for assay development.

CRISPR cell engineering solutions have seamless integration into Multispan’s full assay development, screening and profiling services for drug discovery.

Every year, researchers invest billions of dollars toward clinical approval of innovative new drug compounds. However, the U.S. Federal Drug Administration (FDA) only accepts a select number of new chemical entries every year. In fact, from 2011 to 2018, the FDA only approved 309 new drugs. While this is a marked improvement from the approval rate of decades prior, it still points to the administration’s selectivity. One way to streamline the drug development process? Invest in assay development. This analytical function is key to high-quality drug discovery research. Today, we’re diving into the important role it plays. Ready to learn more? Let’s dive in!

An allosteric modulator binds to an allosteric site on a protein, such as a receptor or enzyme, distinct from the active site, which alters the activity of the protein. This modulation of enzyme and receptor function differs from that of traditional agonists and antagonists. A positive allosteric modulator will enhance the affinity and/or efficacy of a receptor for its endogenous ligand or agonist, and increase the catalytic activity for enzymes. A negative allosteric modulator will reduce the affinity and/or efficacy of the receptor, and decrease the catalytic activity for enzymes.

Assay-Ready Cells For Same-Day Plate-and-Read

Combining Biologically-Relevant Assays with Cell Engineering For GPCR Targets and Beyond