Assay Kits Using MULTISCREEN™ Technology

Validated, high-thoroughput, no-wash, homogenous detection assays

Multispan’s GPCR assay kits, combined with MULTISCREEN™ GPCR stable cell lines and division-arrested cells, truly enable high throughput screening (HTS) and accelerate drug discovery. Moreover, optimized assay protocols using each kit with each cell line developed by our cell and assay experts, add tremendous value to our customer’s experimental setup. These MULTISCREEN™ cross-validated complete solutions allow Multispan experts to assay with confidence in compound screening for customers. Customers can also perform in-house screening with our reagents. 

Product options

MULTISCREEN™ assay kits are developed for high sensitivity, specificity and compatibility for high throughput screening. They are amenable in highly miniaturized settings, in 384-well or higher density microplates. All of Multispan’s assay kits are NO-WASH HOMOGENEOUS assay.

MULTISCREEN™ assay kits are divided into 3 categories, addressing the 3 major and most studied GPCR cell signaling pathways. These 3 cell signaling pathways are measured by their respective intracellular second messengers: calcium, cAMP and beta-arrestin. Each of the 3 types of assay kits employs a different detection mechanism such as fluorescence, TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or chemiluminescence, designed to be best suited for the chemical and molecular nature of each specific second messenger being measured. The different readouts not only maximize the robustness and sensitivity of the assays, but also enable the possibility of multiplexing, ie, two assays in one well. Multiplex assays are in general much desired in HTS assays to save time and cost. 

GPCR assay kits used in compound screening

Distinctive benefits of MULTISCREEN™ assay kits:

  • No-wash, homogenous assays for beta-arrestin, calcium and cAMP detection
  • Complete solution with Multispan’s stable cell lines and division-arrested cells
  • Compatible with 96-, 384-, and higher density plate formats
  • Detailed assay protocols from cells to assays
  • Validated and routinely used by Multispan’s lab in compound screening campaigns
  • Completion solution developed and supported by Mutispan’s cell and assay experts

MULTISCREEN™ β-Arrestin Assay Kits

The properties of a ligand that preferentially activates or inhibits one pathway over another are described as cell signaling bias. Screening compounds for biased cell signaling may lead to selective perturbation of disease-specific pathways. Measuring compound activities in cell assays in the same cellular environment, using recombinant or native unmodified GPCRs, is key for accurate cell signaling bias analysis. Our newly developed proprietary and patented MULTISCREEN™ Beta-Arrestin recruitment assay technology overcomes the receptor-tagging drawback of other technologies, enabling high throughput detection of beta-arrestin translocation induced by native GPCRs in vitro and in vivo for the first time. 

MULTISCREEN™ Beta-Arrestin assay kits provide no-wash homogeneous luminescence assays for intracellular β-Arrestin translocation detection in 96-well, 384-well and higher multiplexity. Total hands-on time for the assay is less than 30 minutes. They are suitable for any cell line or primary cells co-expressing endogenous or untagged GPCR target and MULTISCREEN™ β-Arrestin sensor introduced by viral transfections. 

  • MULTISCREEN™ β-Arrestin Assay Kit: This basic assay kit contains Substrate and Assay Buffer. It’s validated by MULTISCREEN™ stable cell lines co-expressing target GPCRs and MULTISCREEN™ β-Arrestin sensors. The kit is validated by MULTISCREEN™ GPCR stable cell lines and division-arrested cells.
  • MULTISCREEN™ β-Arrestin BacMam Assay Kit: This viral delivery kit contains BacMam viral particles expressing the bi-cistronic MULTISCREEN™ β-Arrestin sensor cDNA, control GFP BacMam viral particle, the Substrate and Assay Buffer. The kit is validated by MULTISCREEN™ GPCR stable cell lines and division-arrested cells.
  • MULTISCREEN™ β-Arrestin HSV Assay Kit: This viral delivery kit contains Herpes Simplex viral (HSV) particles expressing the bi-cistronic MULTISCREEN™ β-Arrestin sensor cDNA, control GFP HSV viral particle, the Substrate and Assay Buffer. The kit is validated by MULTISCREEN™ GPCR stable cell lines and division-arrested cells.

The kits contain the substrate for MULTISCREEN™ β-Arrestin sensors and assay buffers. They provide homogeneous luminescence assays for intracellular β-Arrestin translocation detection in 96-well, 384-well and higher multiplexity. Total hands-on time for the assay is less than 30 minutes.

Validated by MULTISCREEN™ stable cell lines co-expressing target GPCRs and MULTISCREEN™ β-Arrestin sensors. Suitable for any cell line or primary cells co-expressing endogenous or untagged GPCR target and MULTISCREEN™ β-Arrestin sensor.

Unit Size (384-Well) Catalog Number Data
1 plate 96-well/384-well
MSBAK01-1

β-Arrestin Recruitment Dose Response in MULTISCREEN™ MOR β-Arrestin2 CHO-K1 stable cell line (CA1350-1a) measured with MULTISCREEN™ β-Arrestin Assay Kit. Cells were seeded in assay buffer in a 384-well plate, incubated with the MULTISCREEN™ Substrate before being treated with control agonist DAMGO and measured by Flexstation 3.

 

10 plates 96-well/384-well
MSBAK01-10
100 plates 96-well/384-well
MSBAK01-100

The kits contain BacMam viral particles of MULTISCREEN™ β-Arrestin sensor cDNA, control GFP BacMam viral particle, the substrate for MULTISCREEN™ β-Arrestin sensors and assay buffers. They provide homogeneous luminescence assays for intracellular β-Arrestin translocation detection in 96-well, 384-well and higher multiplexity. Total hands-on time for the assay is less than 30 minutes.

Suitable for any cell line or primary cells expressing endogenous or untagged GPCR target.

Unit Size (384-Well) Catalog Number Data
1 plate 96-well/384-well
β-Arrestin1 MSBAKBM01-1
Or
β-Arrestin2 MSBAKBM02-1

β-Arrestin Recruitment Dose Response in MULTISCREEN™ MOR CHO-K1 stable cell line (C1350-1a) infected with β-Arrestin sensor BacMam virus for 48 hours before being measured with MULTISCREEN™ β-Arrestin Assay Kit. Cells were seeded in a 384-well plate, incubated with the MULTISCREEN™ Substrate before being treated with control agonist DAMGO and measured by Flexstation 3.

 

10 plates 96-well/384-well
β-Arrestin1 MSBAKBM01-10
Or
β-Arrestin2 MSBAKBM02-10
100 plates 96-well/384-well
β-Arrestin1 MSBAKBM01-100
Or
β-Arrestin2 MSBAKBM02-100

The kits contain HSV viral particles of MULTISCREEN™ β-Arrestin sensor cDNA, control GFP HSV viral particle, the substrate for MULTISCREEN™ β-Arrestin sensors and assay buffers. They provide homogeneous luminescence assays for intracellular β-Arrestin translocation detection in 96-well, 384-well and higher multiplexity. Total hands-on time for the assay is less than 30 minutes.

Suitable for any cell line or primary cells expressing endogenous or untagged GPCR target.

Unit Size (384-Well) Catalog Number Data
1 plate 96-well/384-well
β-Arrestin1 MSBAKHSV01-1
Or
β-Arrestin2 MSBAKHSV02-1

β-Arrestin Recruitment Dose Response in MULTISCREEN™ KOR HEK293T stable cell line (C1352) infected with β-Arrestin HSV sensor for 48 hours before being measured with MULTISCREEN™ β-Arrestin Assay Kit. Cells were seeded in a 384-well plate, incubated with the MULTISCREEN™ Substrate before being treated with control agonist Dynorphin B and measured by Flexstation 3.

 

10 plates 96-well/384-well
β-Arrestin1 MSBAKHSV01-10
Or
β-Arrestin2 MSBAKHSV02-10
100 plates 96-well/384-well
β-Arrestin1 MSBAKHSV01-100
Or
β-Arrestin2 MSBAKHSV02-100

MULTISCREEN™ Calcium No-Wash Assay Kits

Calcium assay measures one of the major signaling pathways downstream of GPCRs upon ligand binding. MULTISCREEN™ Calcium No Wash Assay Kits provides homogeneous fluorescence-based assays for intracellular calcium mobilization detection in 96-well, 384-well and higher multiplexity. Pre-loaded with Calcium 1.0 dye is taken up by the cells where the lipophilic blocking groups of Calcium 1.0 are cleaved by esterases, resulting in a negatively charged fluorescent dye that stays inside cells. The fluorescence signal is greatly enhanced after binding to intracellular calcium released by cell -stimulation. Long wavelength, high sensitivity, and >100 times fluorescence enhancement make Calcium 1.0 dye an ideal indicator for intracellular calcium release assays measuring GPCRs and calcium channels. MULTISCREEN™ Calcium 1.0 No Wash Assay Kits are validated by MULTISCREEN™ stable cell lines and division-arrested cells. 

MULTISCREEN™ Calcium 1.0 No Wash Assay Kits provide homogeneous fluorescence-based assays for intracellular calcium mobilization detection in 96-well, 384-well and higher multiplexity. Pre-loaded with Calcium 1.0 is taken up by the cells where the lipophilic blocking groups of Calcium 1.0 are cleaved by esterases, resulting in a negatively charged fluorescent dye that stays inside cells. The fluorescence signal is greatly enhanced after binding to intracellular calcium released by cell-stimulation. Long wavelength, high sensitivity, and >100 times fluorescence enhancement make Calcium 1.0 an ideal indicator for intracellular calcium release assays measuring GPCRs, calcium channels and receptor tyrosine kinase signaling.

MULTISCREEN™ Calcium 1.0 No Wash Assay Kits are validated by MULTISCREEN™ stable cell lines.

Unit Size (384-Well) Catalog Number Data
1000 tests
MSCA01-1

Calcium Dose Response in MULTISCREEN™ A2B-HEK293T stable cell line (C1429) measured with MULTISCREEN™ Calcium 1.0 No Wash Assay Kit. Cells were seeded in assay buffer in a 384-well black wall clear bottom PDL-coated plate, incubated with the MULTISCREEN™ Calcium 1.0 No Wash Assay Kit before being treated with control agonist NECA and measured by FLIPR.

10000 tests
MSCA01-10
100000 tests
MSCA01-100

MULTISCREEN™ TR-FRET cAMP No Wash Assay Kits

cAMP assay measures another one of the major signaling pathways downstream of GPCRs upon ligand binding. MULTISCREEN™ TR-FRET cAMP No Wash Assay Kits provide a homogenous TR-FRET assay method for adenylyl cyclase activity detection in 96-well, 384-well and higher multiplexity. cAMP antibody (Ab) is labeled with MULTISCREEN™ Eu while cAMP is labeled with MULTISCREEN™ 650. In the absence of cAMP, MULTISCREEN™ 650-cAMP is bound to MULTISCREEN™ Eu-cAMP-Ab to give a strong TR-FRET emission at 655 nm. Free cAMP in the test sample competes for binding to MULTISCREEN™ Eu-cAMP-Ab, reducing TR-FRET signal from MULTISCREEN™ 650-cAMP binding. The MULTISCREEN™ 650-cAMP only has fluorescence lifetime of nanosecond while MULTISCREEN™ Eu-cAMP-Ab has much longer fluorescence lifetime value due to TR-FRET. The magnitude of TR-FRET is proportional to the concentration of cAMP in a sample. MULTISCREEN™ TR-FRET cAMP 1.0 No Wash Assay Kits are validated by MULTISCREEN™ stable cell lines and division-arrested cells. 

MULTISCREEN™ TR-FRET cAMP 1.0 No Wash Assay Kits provide a homogenous TR-FRET assay method for adenylyl cyclase activity detection in 96-well, 384-well and higher multiplexity. α-cAMP antibody (Ab) is labeled with MULTISCREEN™ Eu while cAMP is labeled with MULTISCREEN™ 650. In the absence of cAMP, MULTISCREEN™ 650-cAMP is bound to MULTISCREEN™ Eu-α-cAMP-Ab to give a strong TR-FRET Emission at 655 nm. Free cAMP in the test sample competes for binding to the MULTISCREEN™ Eu-α-cAMP-Ab, reducing TR-FRET signal from MULTISCREEN™ 650-cAMP binding. The MULTISCREEN™ 650-cAMP only has fluorescence lifetime of nanosecond while MULTISCREEN™ Eu-α-cAMP-Ab has much longer fluorescence lifetime value due to TR-FRET. The magnitude of TR-FRET is proportional to the concentration of cAMP in a sample.

MULTISCREEN™ TR-FRET cAMP 1.0 No Wash Assay Kits are validated by MULTISCREEN™ stable cell lines.

Unit Size (384-Well) Catalog Number Data
500 tests
MSCM01-1

cAMP Dose-Response in MULTISCREEN™ β1-HEK293T stable cell line (C1437-1) measured with MULTISCREEN™ TR-FRET cAMP 1.0 No Wash Assay Kit. Cells were seeded in a 384-well solid white plate, incubated with Isoproterenol and 1mM IBMX before being treated with MULTISCREEN™ Eu-α-cAMP-Ab and MULTISCREEN™ 650-cAMP, and measured by FlexStation 3.

5000 tests
MSCM01-5
25000 tests
MSCM01-25

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