MULTISCREEN^TM cell line development integrates Multispan team’s cell engineering, assay development, and HTS experience and expertise to succeed. Two main categories of cell lines are routinely deployed and engineered by the Multispan team for cell assays.  They are either primary cells expressing the endogenous protein target or stable cell lines that have very low or no expression of endogenous protein target. The isogenic pair of custom MULTISCREEN^TM stable cell lines from the parental cells are engineered by deploying various techniques such as CRISPR knock-in and knock-out, and heterologous recombination target expression. 

To serve the purpose of robust potency assays, we have developed a broad spectrum HTS assays using primary cells such as hepatocytes, hiPSC derived microglial cells, human ventricle interstitial cardiomyocytes (hVIC), primary fibroblasts, PBMC, and mouse islet cells. In the case of heterologous recombination custom stable cell line, we employ Multispan’s highly characterized MULTISCREEN^TMstable cell lines, designed, generated, and QC’ed for high throughput screening assays. These potency assays are either driven by target-induced cell signaling or translational biological events such as cell proliferation.