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- Upon receiving the package, the vials should be immediately transferred from the dry ice shipping container to a liquid nitrogen storage tank. Please note that storing the cells or membranes at -80°C can cause irreversible damage to them.
- For stable cell lines, we recommend banking at least 10-20 vials from one vial you receive from us immediately before any experiments. This will serve as your master working lot at the lowest passage.
Cell lines and membranes are viable indefinitely if handled and stored properly in liquid nitrogen.
37°C and 5% CO2
We always recommend removing the freezing medium prior to initiating the culture. Thaw and culture the cells as instructed in our cell line protocol.
After thawing and plating the cells, the first medium change should be done after the cells attach to the plate or flask overnight. The medium should be changed every 48-72 hours until the cells are ready to be passaged or used in an assay.
Because different researchers use different split ratios, it is difficult to predict how many passages can be obtained with a particular cell line. Our stable cell lines are guaranteed to be stable for 2 months in continuous culture which is approximately 25-30 passages.
NO. Division-arrested cells should not be cultured in any way. The cells should be thawed and plated immediately in assay plates for adherent or suspended cell assays.
For adherent cell assays, Poly-D-Lysine coated plates are recommended to improve cell adherence.
A different FBS source may be used as long as it matches what we recommend. Cell growth and performance may be adversely affected otherwise.
A different cell culture medium source may be used as long as it contains the exact same components and formulation. Cell growth and performance may be adversely affected otherwise.
For laboratories where Penicillin-Streptomycin (pen strep) is routinely applied in cell culture, adding pen strep in cell culture is strongly advised. However, Multispan does not use Penicillin-Streptomycin in our laboratory to allow any contamination to manifest and be discarded early. We take extra measures to maintain our laboratory and cell lines clean and this is a way to monitor that.
Depending on the customer’s preference, assays may be performed in duplicates or triplicates.
Minimum of 10 points to calculate accurate EC50/IC50.
If you know the expected EC50 of your compounds, we recommend testing 5 points higher and 5 points lower than EC50. If your EC50 is unknown, we recommend testing at 10uM top concentration with 5-fold serial dilutions to cover a long-range.
Here is an example of positive and negative data with the treatment of drugs in our calcium assays including full agonist and partial agonist activity when compounds are tested alone, and antagonist activities when compounds are tested in the presence of natural agonist at its EC80 concentration.
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MultiScreen™ TR-FRET cAMP 1.0 No Wash Assay Kit
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MultiScreen™ Calcium 1.0 No Wash Assay Kit
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Tips for Establishing Successful Cell-Based Assays: Part 1
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Tips for Establishing Successful Cell-Based Assays: Part 2
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Tips for Establishing Successful Cell-Based Assays: Part 3
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Strategies and Tools to Develop a Better GPCR Targeted Therapeutic
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Use Functional Assays to Select CRISPR Gene-Edited Stable Cell Lines
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BacMam Viral Delivery of MultiScreen™ β-Arrestin Sensor for Endogenous GPCRs
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Robust Beta-Arrestin Recruitment Assay in Proprietary MultiScreen™ GPCR Stable Cell Lines
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Orphan GPRC5A Stable Cell Line Assay Developed with MultiScreen™ β-Arrestin Sensor Technology
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Viral Delivery of MultiScreen™ β-Arrestin Sensor for Endogenous GPCRs
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β-Arrestin and cAMP Assays for Proton Sensing Receptors
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MultiScreen™ Division-Arrested Cell Lines
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GPR4, GPR65, GPR68, GPR132 Proton Sensing Receptors
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New MultiScreen™ β-Arrestin Sensor for Glucagon and GLP-1 Receptors
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High Quality Membranes for 400 GPCRs
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MultiScreen™ β-Arrestin Sensor for GLP-1 Receptor
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NEW MultiScreen™ Ortholog Sphingosine-1-Phosphate Receptor Stable Cell Lines
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MultiScreen™ β-Arrestin Sensors for Endogenous GPCRs and Primary Cells
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High and Low Expression Glucagon and GLP-1 Receptor MultiScreen™ Stable Cell Lines
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Let Multispan Help with your GPCR Radioligand Binding Screening
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MultiScreen™ β-Arrestin Sensor for Native GPCRs
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MultiScreen™ Calcium-1.0 and TR-FRET cAMP 1.0 No Wash Assay Kit
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NEW Beta-Arrestin Recruitment Technology from Multispan
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NEW Stable Pool Service
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MultiScreen™ Division-Arrested Cell Lines
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NEW GPR35 Stable Cell Lines from Multispan
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NEW Cell-Based Kinase Assays
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Drive SAR Screening Campaign with Rapid Turnaround
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SAR Lead Optimization Routine Screening Support