Learning Is Power
MULTISCREEN™ GPCR DIVISION-ARRESTED CELLS
Do you need to do a few quick experiments to validate your target?
Are you tired of all the variability and inconsistency of cell cultures?
Do you need to a reliable resource of cells for GMP assay validation?
Do you want to decouple the labor-intensive and time-consuming cell line production from your research and screening?
Multispan’s MultiScreen™ division-arrested cells are your solution! MultiScreen™ division-arrested cells enable fast 1-day plate-to-screen workflows with excellent batch-to-batch assay consistency, saving you 6 months of cell line and assay development, cell culture scale-up, and QA/QC work.
MultiScreen™ division-arrested cells shorten time-to-assay
WHAT ARE THEY?
MultiScreen™ GPCR division-arrested cells are derived from our high-quality MultiScreen™ GPCR stable cell lines. They are assay-ready frozen cells intended for plate and read on the same day.
Large scale cell culture is no longer required! MultiScreen™ GPCR division-arrested cells save months of assay development and laborious tissue culture work. They enable you to jumpstart your research for target validation, high-throughput and SAR screening, and compound profiling.
Packed in 4 million-cell single-use cryotubes upon freezing, MultiScreen™ GPCR division-arrested cells are developed for one 384- or 96-well microtiter-plate assays. Therefore, they are low-cost for evaluating a small number of compounds. They can also be used to test tool compounds in pilot projects before committing to purchasing MultiScreen™ GPCR stable cell lines.
PRODUCT OPTIONS Multispan’s division-arrested cells are available in human and ortholog GPCR target pairs with matching pharmacology and expression. Besides 4 million-cell cryovials, they are also available at higher density of billions of cells in bulk aliquots. They can be selected fromm over 500 highly-characterized MultiScreen™ GPCR stable cell lines. Moreover, division-arrested parental cells for each target are available and recommended as negative controls.
Key features of MultiScreen™ division-arrested cells
WHICH ASSAYS? Multispan’s division-arrested cells maintain the multi-screen nature of multiple assays in each MultiScreen™ stable cell line. We bank and validate a new batch of cells in one specific assay requested. As a result, frequently they are validated in calcium assay, cAMP assay, beta-arrestin assay and binding assay by flow cytometry (FACS).
HOW WELL DO THEY PERFORM? Our goal is to obtain pharmacological profiles in our division-arrested cells similar to those in growing cells. Each MultiScreen™ GPCR stable cell line is rigorously validated for assay function, clonality and stability. In addition, to preserve the high performance of our stable cell lines and batch-to-batch consistency, Multispan’s researchers have developed proprietary processes in cell culture scaling up, growth-arrest, freezing and thawing. Each batch of assay-ready cells is QC’ed in a functional assay side-by-side with its original stable cell line.
HOW WELL DO THEY PERFORM? Our goal is to obtain pharmacological profiles in our division-arrested cells similar to those in growing cells. Each MultiScreen™ GPCR stable cell line is rigorously validated for assay function, clonality and stability. In addition, to preserve the high performance of our stable cell lines and batch-to-batch consistency, Multispan’s researchers have developed proprietary processes in cell culture scaling up, growth-arrest, freezing and thawing. Each batch of assay-ready cells is QC’ed in a functional assay side-by-side with its original stable cell line.
MultiScreen™ cAMP assay of 5-HT7 receptor division-arrested cell and stable cell line
IN SUMMARY, you can count on MultiScreen™ division-arrested cells to achieve the excellent assay consistency from batch to batch. This is required by not only GMP assay validation but also high-quality drug discovery and academic research.
Please email info@multispaninc.com for more. Let’s discuss how MultiScreen™ GPCR division-arrested cells can uniquely empower your program.
Recent Articles
MEETING THE NEED FOR ACCELERATED COVID ANTIVIRAL AND VACCINE DEVELOPMENT
The foundational role of relevant, robust cell-based assays in covid antiviral and vaccine development.
Don’t Let GPCR Tagging Bias Your Results
Harness the power of freeing your GPCR from unwanted tags using Multispan’s proprietary MultiScreen™ β-Arrestin sensor technology.
HIGH THROUGHPUT SCREENING DONE RIGHT
The Power of Integrating Screening and Assay Development.
GPCR MEMBRANE LIGAND BINDING ASSAYS THE CRITICAL ROLE OF CELL MEMBRANES
Explore the many types of ligand binding assays using GPCR membranes and the importance of high-quality membranes for assay development.
CRISPR: A NEW BREAK IN THE PIPELINE
CRISPR cell engineering solutions have seamless integration into Multispan’s full assay development, screening and profiling services for drug discovery.
BUILDING BETTER DRUGS
The role assay development plays in drug discovery.
Recent Comments