MULTISCREEN™ GPCR DIVISION-ARRESTED CELLS

Key features of MultiScreen™ division-arrested cells

WHICH ASSAYS? Multispan’s division-arrested cells maintain the multi-screen nature of multiple assays in each MultiScreen™ stable cell line. We bank and validate a new batch of cells in one specific assay requested. As a result, frequently they are validated in calcium assay, cAMP assay, beta-arrestin assay and binding assay by flow cytometry (FACS).

HOW WELL DO THEY PERFORM? Our goal is to obtain pharmacological profiles in our division-arrested cells similar to those in growing cells. Each MultiScreen™ GPCR stable cell line is rigorously validated for assay function, clonality and stability. In addition, to preserve the high performance of our stable cell lines and batch-to-batch consistency, Multispan’s researchers have developed proprietary processes in cell culture scaling up, growth-arrest, freezing and thawing. Each batch of assay-ready cells is QC’ed in a functional assay side-by-side with its original stable cell line.

HOW WELL DO THEY PERFORM? Our goal is to obtain pharmacological profiles in our division-arrested cells similar to those in growing cells. Each MultiScreen™ GPCR stable cell line is rigorously validated for assay function, clonality and stability. In addition, to preserve the high performance of our stable cell lines and batch-to-batch consistency, Multispan’s researchers have developed proprietary processes in cell culture scaling up, growth-arrest, freezing and thawing. Each batch of assay-ready cells is QC’ed in a functional assay side-by-side with its original stable cell line.

MultiScreen™ cAMP assay of 5-HT7 receptor division-arrested cell and stable cell line

IN SUMMARY, you can count on MultiScreen™ division-arrested cells to achieve the excellent assay consistency from batch to batch. This is required by not only GMP assay validation but also high-quality drug discovery and academic research.