Antibody Screening and Profiling, Assay-Ready on Day One
Cell-based binding, function, internalization, and cross-species screening. Assay-ready on day one for GPCR targets.
Validated cell lines
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Ortholog species
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384-well benchmark
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Papers & patents
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PATH ONE
GPCR target
The assay already exists. Screening starts immediately.
Days to first data
600+ validated lines
Ortholog pairs already matched
Custom panels from stock
50-GPCR safety panel available
PATH TWO
Non-GPCR target
We custom build the assay, with a feasibility gate before you commit.
Weeks to first data
Custom cell line generated
Feasibility, then go / no-go
Kinases, ion channels, NHRs
Direct screening saves 6 to 12 months
What do you need to know about your antibody?
Five screening services. Run one or combine them.
Target specificity
Is your antibody specific to your target?
Non-permeabilized staining measures only extracellular binding, normalized for surface expression with anti-Flag. Custom panels built from 600+ validated lines.
Functional screening
Does your antibody activate or inhibit your target?
Calcium, cAMP, β-arrestin and more. For GPCRs, run on untagged receptors via our MULTISCREEN™ HTS assay kits.
Internalization
Does your antibody internalize your target receptor?
Target-induced internalization as a functional readout, measured by live cell flow cytometry.
Cross-species profiling
Do you need a surrogate for animal studies?
Human and ortholog lines side by side, in expression and pharmacology-matched pairs across seven species.
Cytotoxicity
Does your antibody kill the target cell?
In development. Custom killing assays built on the same cell engineering and assay development platform.
Not sure which screen your program needs?
Tell us your target and modality. We will tell you which screens answer your question, and which cell lines already exist.
Target specificity
Is your antibody specific to your target?
Non-permeabilized staining measures only extracellular binding, normalized for surface expression with anti-Flag. Custom panels built from 600+ validated lines.
Functional screening
Does your antibody activate or inhibit your target?
Calcium, cAMP, β-arrestin and more. For GPCRs, run on untagged receptors via our MULTISCREEN™ HTS assay kits.
Internalization
Does your antibody internalize your target receptor?
Target-induced internalization as a functional readout, measured by live cell flow cytometry.
Cross-species profiling
Do you need a surrogate for animal studies?
Human and ortholog lines side by side, in expression and pharmacology-matched pairs across seven species.
Cytotoxicity
Does your antibody kill the target cell?
In development. Custom killing assays built on the same cell engineering and assay development platform.
Not sure which screen your program needs?
Tell us your target and modality. We will tell you which screens answer your question, and which cell lines already exist.
EXAMPLE DATA
One antibody. Forty-three receptors.
A commercial anti-CCR5 monoclonal, profiled in our standard FACS assay against 43 GPCRs, including five other chemokine receptors. Cross-reactivity extended well beyond the chemokine family.
"Multispan helped me obtain a much needed cell line very fast. The cell line worked perfectly and helped me to screen antibodies quickly with very little wait and cost."
Jon W, Ph.D.
Principal Scientist, Kadmon
"Fantastic turn-around time for obtaining a great catalog of cell lines and assay services. Results are always well-controlled and with great accuracy..."
Michael Weiner, Ph.D.
VP Molecular Sciences, Abcam
The quality of an antibody lead is only as good as the assays used to select it.