MultiScreen™ Beta-Arrestin Sensor

high throughput detection of biased cell signaling

of GPCRs in MultiScreen™ or primary cells

MultiScreen™ Beta-Arrestin Sensor technology

The properties of a ligand that preferentially activates or inhibits one pathway over another are described as cell signaling bias. Screening compounds for biased cell signaling may lead to selective perturbation of disease-specific pathways. Measuring compound activities in cell assays in the same cellular environment, using recombinant or native unmodified GPCRs, is key for accurate cell signaling bias analysis. Our newly developed proprietary MultiScreen™ Beta-Arrestin recruitment assay technology overcomes the receptor-tagging drawback of other technologies, enabling high-throughput detection of beta-arrestin translocation induced by native GPCRs in vitro and in vivo for the first time.

Illustration of beta-arrestin recruitment assay using Multispan's proprietary Beta-Arrestin Sensors
Simple Protocol for MultiScreen™ Beta-Arrestin Assay
Multispan's Simple Beta-Arrestin Assay Protocol

Multispan uses a proprietary technology to enable expression of GPCR proteins in mammalian cells at over a million molecule per cell surface. High expressing cells(HEx™) can be developed into single cell clones and be used as unique source of high quality GPCR proteins for variety of applications:

  • Antibody generation using antigens with natural epitopes at high expression level
  • Cell-based or membrane receptor binding assays to achieve high signal-to-noise ratio
  • Cell-based functional assays for hard-to-express GPCRs from all 3 structurally distinctive GPCR families
  • Structural studies as high quality source of protein for crystallization