Cell Engineering, Assay Development & Screening Services
Customized, High-quality, Rapid-turnaround Outsourcing Solutions
The Multispan Difference — our collaborative approach, dedication to customer success, commitment to quality, and
breadth of assay experience & target knowledge — enables our clients to bring better medicines to market faster and
more cost-effectively by providing high quality, relevant data.
Multispan Services
Outsourcing solutions to drive new drugs, therapies, and vaccines through the development pipeline.
Cell Line Generation
- Transiently or stably transfected cells to support target discovery to GMP manufacturing assays
- Minimally modified receptors and host cells
- Multispan’s propriety universal MultiScreen™ expression vector is optimized for robust cell surface expression of any transmembrane proteins
- Single cell cloning expertise with 100% successful delivery track record
- Assay-ready: screened using functional assays, delivered with assay protocol
- Comparable stable cell line clones: wildtype vs mutant, ortholog vs human
- Rigorously QC’ed by testing for mycoplasma, RT-PCR sequencing, expression by FACS, and functional assays
Not all cell lines are created equal. Find out why Multispan cell lines outperform.
CRISPR Cell Engineering Solutions
- Stable pool or clonally derived cell line generation
- Clone screening:
- Heterozygous or homozygous stable cell line selection by function
- Selection against parental or orthogonal cell lines
- Cell line validation using real-time PCR and FACS
- Expansion and banking of CRISPR stable cell lines, including production of assay ready, division-arrested cells
- Seamless integration into Multispan’s full assay development, screening, and profiling
services for accelerated drug discovery
Harness the power of CRISPR Engineering. Find out how Multispan solutions ensure production of high quality CRISPR tools.
Assay Development
- Development of customized second messenger, reporter, phenotypic and binding assays in recombinant or primary cells
- Select from the most relevant biological assay to meet screening research needs such as calcium, cAMP, β-arrestin, pGRK2, IP-1, pERK, pNFκB, radioligand binding, GTPγS, chemotaxis, insulin secretion, cell proliferation, luciferase reporter and internalization
- Comparable pharmacology for human and ortholog
targets - Rapid 1-month turnaround time
Accelerating the fight against COVID 19. Find out about the foundational role of relevant, robust cell-based assays.
Screening & Profiling Assay Services
- Human and ortholog GPCRs and beyond in agonist, antagonist, PAM and NAM modes
- Relevant signaling, binding or phenotypic assays in recombinant or endogenous primary cells
- HTS, lead optimization support, primary and secondary screens
- 32-GPCR safety/liability panel implicated in central nervous, cardiac, pulmonary and GI concerns
- 75-oprhan GPCR Calcium assay panel
- Provide reports, analysis and raw data at no extra charge
- Exceptional next-day rapid turnaround for SAR routine screen
The power of integrating assay development and screening. Find out how Multispan solutions ensure HTS is done right.
GPCR Antibody Profiling
There are 350 “druggable” GPCRs which share many similar primary and conformational epitopes. Is your antibody lead SPECIFIC to your GPCR target of interest?
- Assess specificity of the antibody lead in Multispan’s large GPCR expression panel
- Measure antibody binding to GPCRs in native conformations directly on mammalian cell surface by FACS
- Rapid turnaround for shortened lead development cycle
Frozen Cell Scale Up & Banking
Are cell culture activities holding up the development of your therapeutic? Eliminate laborious cell culture in HTS, lead optimization, and manufacturing QC.
- Same day seeding and screening
- Functionally validated
- Grown from master cell bank with the lowest passage number
- MultiScreen™ stable cell lines or cells from a third party
- Scaled up to 0.5-10 billion cells and packaged in 10-50 million cells per frozen vial
- 2-4 week turnaround