Assay Development, Screening Services, & Cell Engineering
Outsourcing solutions to drive new drugs, therapies, and vaccines through the development pipeline.
Assay Development, Screening Services, & Cell Engineering
Outsourcing solutions to drive new drugs, therapies, and vaccines through the development pipeline.
Assay Development, Screening Services, & Cell Engineering
Outsourcing solutions to drive new drugs, therapies, and vaccines through the development pipeline.
Screening & Profiling Assay Services
- HTS, lead optimization support, primary and secondary screens, including for β-Arrestin assays
- Human and ortholog GPCRs and beyond in agonist, antagonist, PAM and NAM modes
- Relevant signaling, binding or phenotypic assays in recombinant or endogenous primary cells
- 32-GPCR safety/liability panel implicated in central nervous, cardiac, pulmonary and GI concerns
- 50-GPCR+ Safety/Liability Functional Assay Panel
- 75-oprhan GPCR Calcium assay panel
- 75-oprhan β-Arrestin assay panel
- 235-GPCR panel screening
Assay Development
- Development of customized second messenger, reporter, phenotypic and binding assays in recombinant or primary cells
- Select from the most relevant biological assay to meet screening research needs such as calcium, cAMP, β-arrestin, pGRK2, IP-1, pERK, pNFκB, radioligand binding, GTPγS, chemotaxis, insulin secretion, cell proliferation, luciferase reporter and internalization
- Development of MULTISCREEN™ β-Arrestin HTS assays and all secondary and tertiary assays required from target to IND
- Comparable pharmacology for human and ortholog targets
- Rapid 1-month turnaround time
Cell Line Generation
- Transiently or stably transfected cells to support target discovery to GMP manufacturing assays
- Minimally modified receptors and host cells
- Multispan’s propriety universal MULTISCREEN™ expression vector is optimized for robust cell surface expression of any transmembrane proteins
- Single cell cloning expertise with 100% successful delivery track record
- Assay-ready: screened using functional assays, delivered with assay protocol
- Comparable stable cell line clones: wildtype vs mutant, ortholog vs human
- Rigorously QC’ed by testing for mycoplasma, RT-PCR sequencing, expression by FACS, and functional assays
CRISPR Cell Engineering Solutions
- Stable pool or clonally derived cell line generation
- Clone screening:
- Heterozygous or homozygous stable cell line selection by function
- Selection against parental or orthogonal cell lines
- Cell line validation using real-time PCR and FACS
- Expansion and banking of CRISPR stable cell lines, including production of assay ready, division-arrested cells
- Seamless integration into Multispan’s full assay development, screening, and profiling services for accelerated drug discovery
GPCR Antibody Profiling
There are 350 “druggable” GPCRs which share many similar primary and conformational epitopes. Is your antibody lead SPECIFIC to your GPCR target of interest?
Learn More - Assess specificity of the antibody lead in Multispan’s large GPCR expression panel
- Measure antibody binding to GPCRs in native conformations directly on mammalian cell surface by FACS
- Rapid turnaround for shortened lead development cycle
Frozen Cell Scale Up & Banking
Are cell culture activities holding up the development of your therapeutic? Eliminate laborious cell culture in HTS, lead optimization, and manufacturing QC.
Learn More - Same day seeding and screening
- Functionally validated
- Grown from master cell bank with the lowest passage number
- MULTISCREEN™ stable cell lines or cells from a third party
- Scaled up to 0.5-10 billion cells and packaged in 10-50 million cells per frozen vial
- 2-4 week turnaround
The Multispan Difference
Our collaborative approach, dedication to customer success, commitment to quality, and
breadth of assay experience & target knowledge.
We enable our clients to bring better medicines to market faster and
more cost-effectively by providing high quality, relevant data.