MultiScreen™ β-Arrestin Sensor

high throughput detection of biased signaling

of GPCRs in MultiScreen™ or primary cells

MultiScreen™ β-Arrestin technology

The properties of a ligand that preferentially activate or inhibit one pathway over another are described as signaling bias and screening compounds for biased signaling may lead to selective perturbation of disease-specific pathways. Measuring compound activities in assays in the same cellular environment using unmodified GPCRs, recombinant or native, is key for accurate signaling bias analysis. Our newly developed proprietary MultiScreen™ β-Arrestin technology overcomes the receptor-tagging drawback of other existing technologies, enabling high-throughput detection of β-Arrestin translocation induced by native GPCRs in vitro and in vivo for the first time.

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Multispan uses a proprietary technology to enable expression of GPCR proteins in mammalian cells at over a million molecule per cell surface. High expressing cells(HEx™) can be developed into single cell clones and be used as unique source of high quality GPCR proteins for variety of applications:

  • Antibody generation using antigens with natural epitopes at high expression level
  • Cell-based or membrane receptor binding assays to achieve high signal-to-noise ratio
  • Cell-based functional assays for hard-to-express GPCRs from all 3 structurally distinctive GPCR families
  • Structural studies as high quality source of protein for crystallization