MULTISCREEN™ Calcium No-Wash Assay Kits

Homogeneous fluorescence-based assays for intracellular calcium mobilization detection in 96-well, 384-well and higher multiplexity. For GPCRs, calcium channels, and receptor tyrosine kinase signaling.
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Why MULTISCREEN™ Calcium Assay Kits?

Product Features
  • No-wash, homogeneous fluorescence. Designed for HTS workflows.
  • >100 times fluorescence enhancement. High sensitivity for calcium detection.
  • Long wavelength readout. Minimizes interference.
  • HTS-ready. Validated in 96-, 384-, and higher-density formats.
  • Suitable for GPCRs, calcium channels, and receptor tyrosine kinase signaling.
  • Validated with MULTISCREEN™ stable cell lines and division-arrested cells.
Unit Size (384-Well) Catalog Number
1000 tests
MSCA01-1
10000 tests
MSCA01-10
100000 tests
MSCA01-100
MULTISCREEN™ Calcium 1.0 No Wash Assay Kits provide homogeneous fluorescence-based assays for intracellular calcium mobilization detection in 96-well, 384-well and higher multiplexity. Calcium assay measures one of the major signaling pathways downstream of GPCRs upon ligand binding.
Pre-loaded Calcium 1.0 dye is taken up by the cells, where the lipophilic blocking groups of Calcium 1.0 are cleaved by esterases, resulting in a negatively charged fluorescent dye that stays inside cells. The fluorescence signal is greatly enhanced after binding to intracellular calcium released by cell stimulation.
MULTISCREEN™ Calcium 1.0 No Wash Assay Kits are validated by MULTISCREEN™ stable cell lines and division-arrested cells.
Calcium Dose Response in MULTISCREEN™ A2B-HEK293T stable cell line (C1429) measured with MULTISCREEN™ Calcium 1.0 No Wash Assay Kit. Cells were seeded in assay buffer in a 384-well black wall clear bottom PDL-coated plate, incubated with the MULTISCREEN™ Calcium 1.0 No Wash Assay Kit before being treated with control agonist NECA and measured by FLIPR.

Structure-Based Drug Design of ADRA2A Antagonists Derived from Yohimbine

GPCR Target: Calcium assay

Authors: Chayka A, et al.

Journal: J Med Chem. 2024 Jun 27;67(12):10135-10151. doi: 10.1021/acs.jmedchem.4c00323.

Year Published: 2024

New quinolone derivatives as neuropeptide S receptor antagonists: Design, synthesis, homology modeling, dynamic simulations and modulation of Gq/Gs signaling pathways.

GPCR Target: Calcium assay, cAMP assay

Authors: Batran RZ, Gugnani KS, Maher TJ, Khedr MA.

Company: Chemistry of Natural Compounds Department, Pharmaceutical and Drug Industries Research Division, National Research Centre,Dokki, Giza, Egypt; Department of Pharmaceutical Sciences, MCPHS University, Boston, MA, USA.; Pharmaceutical Chemistry Department, Faculty of Pharmacy, Helwan University, Ein Helwan, Cairo, Egypt

Journal: Bioorg Chem. 2021 Jun;111:104817

Year Published: 2021

Distinct biological activities of isomers from several families of branched fatty acid esters of hydroxy fatty acids (FAHFAs). 

GPCR Target: Calcium assay

Authors: Aryal P, Syed I, Lee J, Patel R, Nelson AT, Siegel D, Saghatelian A, Kahn BB

Company: Division of Endocrinology, Diabetes and Metabolism, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA; Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, California, USA; Clayton Foundation Laboratories for Peptide Biology, Salk Institute for Biological Studies, La Jolla, California, USA; Division of Endocrinology, Diabetes and Metabolism, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA

Journal: J Lipid Res. 2021;62:100108.

Year Published: 2021

In Vitro Pharmacological Profile of a New Small Molecule Bradykinin B2 Receptor Antagonist

GPCR Target: Calcium assay

Authors: Anne Lesage, et al.

Company: Pharvaris Netherlands B.V., Leiden, Netherlands

Journal: Frontiers in Pharmacology. June 19, 2020.

Year Published: 2020

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