Product Information
Catalog Number:
DCm1104
Lot Number:
DCm1104-030524
Quantity:
1 vial (4 x 106) frozen cells
Freeze Medium: Cellbanker 2
Host cell:
HEK293T
Transfection: Expression vector containing full-length mouse FFAR2 cDNA (GenBank Accession Number NM_146187.4) with FLAG tag sequence at N-terminus
Recommended Storage:
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
Data Sheet
Background: GPR43, or free fatty acid receptor 2 (FFAR2), encodes a deduced 330-amino acid protein with 7 transmembrane domains. GPR43 is expressed by white adipose tissue and immune tissue of adult mice. The receptor binding of short-chain fatty acids potentially provides an intestine molecular link between diet, gastrointestinal bacterial metabolism, and immune and inflammatory responses.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MULTISCREEN™ Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
References:
Kimura, I. et al. The gut microbiota suppresses insulin-mediated fat accumulation via the short-chain fatty acid receptor GPR43. Nat. Commun. 4:1829 doi: 10.1038/ncomms2852 (2013).
Sawzdargo et al. (1997) Cluster of four novel human G protein-coupled receptor genes occurring in close proximity to CD22 gene on chromosome 19q13.1. Biochem Biophys Res Commun 239:543-547.