HUMAN RECOMBINANT TMEM175 RECEPTOR
MULTISCREENTM STABLE CELL LINE
Catalog Number: C1527a
Lot Number: C1527a-062321
Quantity: 1 vial (2 x 106) frozen cells
Freeze Medium: Cellbanker 2
Host cell: HEK293T
Transfection: Expression vector containing full-length human TMEM175 cDNA (GenBank Accession Number NM_032326.3) with FLAG tag sequence at N-terminus
Recommended Storage: Liquid nitrogen upon receiving
Propagation Medium: DMEM,10% FBS, 1 μg/mL puromycin
Stability: In progress
Background: Transmembrane Protein 175 (TMEM175) is an organelle-specific potassium channel specifically responsible for potassium conductance in endosomes and lysosomes. TMEM175 is reported to regulate lumenal pH stability and is required for autophagosome-lysosome fusion. TMEM175 comprises a K+ channel that underlies the molecular mechanism of lysosomal K+ permeability
Application: Functional assays
Figure 1. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Chunlei Cang et al. “TMEM175 Is an Organelle K(+) Channel Regulating Lysosomal
Function.”Cell 162: 1101–1112. 27 Aug. 2015.