MULTISCREEN™ STABLE CELL LINES
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human SMO cDNA (GenBank Accession Number: NM_005631.3) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stable in culture for minimum of two months
Background: The human smoothened homolog SMO strongly resembles that of the frizzled family of proteins, the receptors of the Wnt signaling pathway, which share several features with the Hedgehog pathway. Most often SMO functions during embryonic development, in processes such as digit patterning in the chick limb bud and left–right asymmetry of vertebrate embryos. In addition, SMO function is fundamental for the maintenance of tissue homeostasis in adults, and deregulated SMO signaling is implicated in tumorogenesis.
Application: Binding assays
Figure Legend. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody
Ruiz-Gómez et al. (2007) The cell biology of Smo signaling and its relationships with GPCRs. Biochim Biophys Acta 1768:901-912.