Product Information
Catalog Number:
MC1054-1
Lot Number:
MC1054-1-082815
Quantity:
1 vial (5.49 mg/mL), 1mg or 5mg
Packaging Buffer: 20mM Gly-Gly, 1 mM MgCl2, 25mM Sucrose (pH 7.2)
Host cell:
CHO-K1
Transfection:
Expression vector containing full-length human S1P5 cDNA (GenBank Accession Number: NM_030760) with FLAG tag sequence at N-terminus
Recommended Storage:
Liquid nitrogen upon receiving. Avoid repeated freeze-thaw.
Data Sheet
Background: The lysosphingolipid sphingosine 1-phosphate (S1P) regulates cell proliferation, apoptosis, motility, and neurite retractions. S1P and the structurally related LPA signal cells through a set of G-protein-coupled receptors known as EDG receptors. S1P5 or EDG8 is a 400-amino acid 7 transmembrane protein that shares 42-49% amino acid identity with the human S1P receptors EDG1, EDG3, and EDG5. Northern blot analysis has shown widespread expression of EDG8 in rat tissues, with strongest expression in spleen and white matter tracts of the brain.
Application: Functional assay
Figure 1. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
References:
IM et al. (2000) Characterization of a novel phingosine 1-phosphate receptor, Edg-8. J Biol Chem 275:14281-14286.