HUMAN RECOMBINANT S1P3 RECEPTOR
MULTISCREEN™ DIVISION-ARRESTED CELL LINE
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human S1P3 cDNA (Genbank Accession Number NM_005226.2) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM/F12, 10% FBS
Stability: Stable for 1-2 days after thawing
Background: S1P3 (sphingosine 1-phosphate receptor 3) or EDG3 (endothelial differentiation G-protein coupled receptor 3) is a receptor for the lysosphingolipid sphingosine 1-phosphate, a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. EDG receptors mediate proangiogenic activities, such as endothelial cell proliferation, chemotaxis, and vessel morphogenesis. S1P3, along with S1P1, is required for directing the organization of endothelial cells into capillary networks.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with Multiscreen™ Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
Takuwa et al. (2001) Subtype-specific, differential activities of the EDG family receptors for sphingosine-1-phosphate, a novel lysophospholipid mediator. Mol Cell Endocrinol 177:3-11.