Product Information
Catalog Number:
DC1051-1
Lot Number:
DC1051-1-012723
Quantity:
1 vial (4 x 106) frozen cells
Freeze Medium:
Cellbanker 2
Host cell:
CHO-K1
Transfection:
Expression vector containing full-length human S1P2 cDNA (Genbank Accession Number NM_005226.2) with FLAG tag sequence at N-terminus
Recommended Storage:
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
Data Sheet
Background: S1P2 (sphingosine 1-phosphate receptor 2) or EDG3 (endothelial differentiation G-protein coupled receptor 2) is a receptor for the lysosphingolipid sphingosine 1-phosphate, a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. EDG receptors mediate pro- angiogenic activities, such as endothelial cell proliferation, chemotaxis, and vessel morphogenesis. S1P2, along with S1P1, is required for directing the organization of endothelial cells into capillary networks.
Application: Functional assays
Figure 1. Dose-dependent stimulation of intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01).
References:
Takuwa et al. (2001) Subtype-specific, differential activities of the EDG family receptors for sphingosine-1-phosphate, a novel lysophospholipid mediator. Mol Cell Endocrinol 177:3-11.