HUMAN RECOMBINANT RXFP2 RECEPTOR
MULTISCREEN™ MEMBRANE PREPARATION
1 vial (19.8mg/mL, 1mg)
20 mM Tris-HCl (pH7.5), 2 mM EDTA, protease inhibitor cocktail
Expression vector containing full-length human RXFP2 cDNA (GenBank accession number: NM_021634)
Store at -80°C. Avoid repeated freeze-thaw.
Background: The relaxin family peptide receptor RXFP2, also known as LGR8, is a 754-amino acid 7 transmembrane protein. RT-PCR detected RXFP2 expression in various organs of the reproductive system along with the brain, kidney, thyroid, muscle, peripheral blood cell, and bone marrow. RXFP2 is known to be important in supporting germ cell function in the testis and ovaries. Additionally, RXFP2 mutations are linked to osteoporosis in men, suggesting that it plays a central role in bone physiology.
Application: Functional assays
Figure 1. Dose-dependent increase of intracellular cAMP level upon treatment with ligand, measured with cAMP HiRange kit (Cisbio 62AM6PEC) using RXFP2 stable cells.
Hall et al. (2006) Relaxin family peptide receptors, RXFP1 and RXFP2, modulate cAMP signalling by distinct mechanisms.. Mol Pharmacol (1):214-26.
Kumagi et al. (2002) INSL3/Leydig insulin-like peptide activates the LGR8 receptor important in testis descent.. J Biol Chem 277: 31283-31286.