HUMAN RECOMBINANT PK2 RECEPTOR, MUTANT
MULTISCREEN™ STABLE CELL LINES
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C- 6164)
Expression vector containing human PROKR2 cDNA (GenBank Accession Number: NM_144773.2) with FLAG tag sequence at N-terminus and 15 bp truncated at the C-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM-F12, 10% FBS, 10 μg/mL puromycin
Stable in culture for minimum of two months
Background: Prokineticin receptor 2 (PK2 or GPR73L1) is a receptor for the cysteine-rich secreted peptides prokineticin 1 and 2. RT-PCR detected PK2 expression in the brain, testis, small intestine, ovary, thyroid, pituitary and salivary gland.
The Multispan PK2 mutant cell line stably expresses the human PROKR2 with the last five amino acids truncated at the C-terminus.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Dose response of intracellular IP1 accumulation upon treatment with ligand, measured with IP-one Tb kit (Cisbio 62IPAPEC). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Lin et al. (2002) Identification and molecular characterization of two closely related G protein-coupled receptors activated by prokineticins/endocrine gland vascular endothelial growth factor. J Biol Chem 277:19276-19280.