HUMAN RECOMBINANT NPY4 RECEPTOR
MULTISCREEN™ DIVISION ARRESTED CELL LINE
1 vial (4x 106) frozen cells
Freeze Medium: Cellbanker 2
Expression vector containing full-length human Y4 cDNA (GenBank Accession Number AY268432) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM-F12, 10% FBS
Stability: Stable for 1 – 2 days after thawing
Background: NPY4R (Y4) has been reported to be expressed at highest levels in brain, coronary artery and ileum, low levels in pancreas and kidney. It is also been detected in colon and small intestine and isolated from human colon and head/neck libraries. It is one of the receptors for neuropeptide Y and peptide YY. The rank order of affinity of this receptor for pancreatic polypeptides is PP, PP (2-36) and [Ile-31, Gln-34] PP > [Pro-34] PYY > PYY and [Leu-31, Pro-34] NPY > NPY > PYY (3-36) and NPY (2-36) > PP (13-36) > PP (31-36) > NPY free acid. Ligand: Neuropeptide Y, Peptide YY.
Application: Functional assay
Figure 1. Dose-dependent calcium flux upon treatment with ligand, measured with MULTISCREENTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
Bard et al. (1995) Cloning and functional expression of a human Y4 subtype receptor for pancreatic polypeptide, neuropeptide Y, and peptide YY. J Biol Chem 270:26762- 26765.
Yan et al. (1996) Cloning and functional expression of cDNAs encoding human and rat pancreatic polypeptide receptors. Proc Natl Acad Sci USA 93:4661-4665.