Product Information
Catalog Number:
DC1305
Lot Number:
DC1305-020422
Quantity:
1 vial (4 x 106) frozen cells
Freeze Medium:
Cellbanker 2 (Amsbio)
Host cell:
HEK293T
Transfection:
Expression vector containing full-length human TACR3 cDNA (GenBank Accession Number NM_001059.1) with FLAG tag sequence at N-terminus
Recommended Storage:
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
Data Sheet
Background: Three membrane receptors that recognize these tachykinins have been described, termed TACR1, TACR2, and TACR3 (or NK3). Their endogenous ligands are substance P, neurokinin A, and neurokinin B, respectively. Interest in the tachykinins and their respective receptors has been stimulated by their potential role in bronchial asthma.
Application: Functional assays
Figure 1. Dose-dependent calcium flux upon treatment with ligand, measured with MULTISCREEN™ Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
References:
Hopkins et al. (1991) Isolation and characterisation of the human lung NK-1 receptor cDNA. Biochem Biophys Res Commun 180:1110-1117.
Huang et al. (1992) cDNA sequence and heterologous expression of the human neurokinin-3 receptor. Biochem Biophys Res Commun 184:966-972.