MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT αVβ1 RECEPTOR
1 vial (2 x 106) frozen cells
Expression vector containing full-length human ITGAV cDNA (GenBank accession number NM_002210.4) and full-length human ITGB1 (GenBank accession number NM_002211.3)
Liquid nitrogen upon receiving
Propagation Medium: DMEM/F12, 10% FBS, 10 µg/mL puromycin, 250 µg/mL hygromycin
Stable for a minimum of 2 months in continuous culture
Background: αVβ1 integrins are transmembrane heterodimeric receptors that contribute to diverse biological functions and play critical roles in many human diseases. αVβ1 is highly expressed on activated fibroblasts, directly binds to the latency-associated peptide of transforming growth factor–b1 (TGFb1) and mediates TGFb1 activation.
Application: Functional assays
Figure 1. Receptor expression on cell surface measured by flow cytometry (FACS) using using CD51 antibody (a) and CD29 antibody (b). Thin line: parental cells; thick line: receptor-expressing cells.
Nilgun I. Reed, et al. (2003) The avb1 integrin plays a critical in vivo role in tissue fibrosis. Science Translational Medicine. 20 May 2015 Vol 7 Issue 288 288ra79