MULTISCREEN™ STABLE CELL LINES

HUMAN RECOMBINANT αVβ1 RECEPTOR

Product Information

Catalog Number:
C3001-1

Lot Number:
C3001-1-45B-071717

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Cellbanker 2

Host cell:
CHO B2

Transfection:
Expression vector containing full-length human ITGAV cDNA (GenBank accession number NM_002210.4) and full-length human ITGB1 (GenBank accession number NM_002211.3)

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM/F12, 10% FBS, 10 µg/mL puromycin, 250 µg/mL hygromycin

Stability:
Stable for a minimum of 2 months in continuous culture

Data Sheet

Background: αVβ1 integrins are transmembrane heterodimeric receptors that contribute to diverse biological functions and play critical roles in many human diseases. αVβ1 is highly expressed on activated fibroblasts, directly binds to the latency-associated peptide of transforming growth factor–b1 (TGFb1) and mediates TGFb1 activation.

Application: Functional assays

Figure 1. Receptor expression on cell surface measured by flow cytometry (FACS) using using CD51 antibody (a) and CD29 antibody (b). Thin line: parental cells; thick line: receptor-expressing cells.

References:

Nilgun I. Reed, et al. (2003) The avb1 integrin plays a critical in vivo role in tissue fibrosis. Science Translational Medicine. 20 May 2015 Vol 7 Issue 288 288ra79

FOR RESEARCH USE ONLY.
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