MULTISCREEN™ STABLE CELL LINES

HUMAN RECOMBINANT MRGX2 RECEPTOR

Product Information

Catalog Number:
C1257a

Lot Number:
C1257a-122017

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Cell Banker 2 (Amsbio 11891)

Host cell:
HEK293T

Transfection:
Full-length human MRGX2 cDNA (GenBank Accession Number NM_054030) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin

Stability:
In progress

Data Sheet

Background: MRGX2 (MAS-related GPR member X2) is widely known as a receptor for cortistatin. It is probably involved in the function of nociceptive neurons and regulation of nociceptor function and/or development, including the sensation or modulation of pain. Cortistatin-14, a high potency agonist at the receptor, has biological functions including sleep regulation, locomotor activity and cortical function. Recent studies have found that MrgX2 is also a potential human PAMP-12 receptor that acts by inhibiting of the forskolin-elevated cAMP accumulation and regulates catecholamine secretion from adrenal glands.

Application: Functional assays

Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP accumulation upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Robas et al. (2003) MrgX2 is a high potency cortistatin receptor expressed in dorsal root ganglion. J Biol Chem 278:44400-44404.

Kamohara et al. (2005) Identification of MrgX2 as a human G-protein-coupled receptor for proadrenomedullin N-terminal peptides. Biochem Biophys Res Commun 330:1146-1152.

FOR RESEARCH USE ONLY.
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