HUMAN RECOMBINANT MRGX2 RECEPTOR
MULTISCREEN™ DIVISION ARRESTED CELL LINE
1 vial (4 x 106) frozen cells
Cell banker 2
Full-length human MRGX2 cDNA (GenBank Accession Number NM_054030)
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
Stable 1-2 days after thawing
Background: MRGX2 (MAS-related GPR member X2) is a receptor for cortistatin. It is probably involved in the function of nociceptive neurons and regulation of nociceptor function and/or development, including the sensation or modulation of pain. Cortistatin-14, a high potency agonist at the receptor, has biological functions including sleep regulation, locomotor activity and cortical function.
Application: Functional assays
Figure Legend: Dose-dependent stimulation of calcium flux upon treatment with ligand, monitored with FLIPR.
Robas et al. (2003) MrgX2 is a high potency cortistatin receptor expressed in dorsal root ganglion. J Biol Chem 278:44400-44404.
Kamohara et al. (2005) Identification of MrgX2 as a human G-protein-coupled receptor for proadrenomedullin N-terminal peptides. Biochem Biophys Res Commun 330:1146-1152.