Product Information
Catalog Number:
C1366
Lot Number:
C1366-082225
Quantity:
1 vial (2 x 106) frozen cells
Freeze Medium:
Cellbanker 2
Host cell:
HEK293T
Transfection:
Expression vector containing full-length human MrgD cDNA (GenBank accession number NM_198923.1) with FLAG tag sequence at N-terminus
Recommended Storage:
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stability:
In progress
Data Sheet
Background: MRGD (MRGPRD) is a Class A Mas‑related G protein‑coupled receptor predominantly expressed in subsets of peripheral sensory neurons, where it contributes to the detection of noxious and pruritic stimuli, including β‑alanine‑evoked itch and pain‑related signaling. Experimental studies show that MRGPRD responds to endogenous small‑molecule agonists such as β‑alanine in functional screens, engaging G protein–mediated pathways to modulate intracellular Ca²⁺ and sensory neuron excitability. Its expression in dorsal root ganglia and mechanistic linkage to somatosensory processes underscores its relevance as a target in pain, itch, and inflammatory research.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MULTISCREEN™ Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Black line: parental cells; gray line: receptor-expressing cells.
References:
Suzuki S, Iida M, Hiroaki Y, et al. (2022) Structural insight into the activation mechanism of MrgD with heterotrimeric Gi-protein revealed by cryo-EM. Commun Biol 5:707.
Uno M, Nishimura S, Fukuchi K, et al. (2012) Identification of physiologically active substances as novel ligands for MRGPRD. J Biomed Biotechnol 2012:816159.
Wang H, Zylka MJ. (2009) Mrgprd-expressing polymodal nociceptive neurons innervate most known classes of substantia gelatinosa neurons. J Neurosci 29:13202-13209.