HUMAN RECOMBINANT MGLUR6 RECEPTOR
MULTISCREEN™ STABLE CELL LINES
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human GRM6 cDNA (NM_000843.3) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM with GlutaMAX (Gibco 10566), 10% FBS (dialyzed), 2 mM sodium pyruvate, 1 μg/mL puromycin
Stable after two months of continuous growth
Background: The metabotropic glutamate receptors (mGluRs) are divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacological properties. Group II and group III mGluRs are linked to inhibition of the cyclic AMP cascade, but differ in their agonist selectivity. mGluR6 is a member of Group III. It mainly expresses in on-bipolar cells in retina.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Dose- dependent response of forskolin-stimulated intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Laurie et al. (1997) Cloning, distribution and functional expression of the human mGlu6 metrbottropic glutamate receptor. Neuropharmacology 36:145-152.
Dryja et al. (2005) Night blindness and abnormal cone electroretinogram on responses in patients with mutations in the GRM6 gene encoding mGluR6. Proc Natl Acad Sci USA 102:4884–4889.