Product Information
Catalog Number:
DC1050-6
Lot Number:
06/21/13
Quantity:
1 vial (2 x 106) frozen cells
Freeze Medium:
Sigma Freezing Medium (C-6164)
Host cell:
RH7777
Transfection:
Expression vector containing full-length human EDG4 cDNA (GenBank Accession Number NM_004720.4) with FLAG tag sequence at N-terminus
Recommended Storage:
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
Stability:
Stable for 1-2 days after thawing
Data Sheet
Background: The lysophosphatidic acid receptor LPA2 or endothelial differentiation, G-protein coupled receptor 4 (EDG-4) is expressed most abundantly in testes and peripheral blood leukocytes. It is reported to be a distinctive functional marker for ovarian carcinoma.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
References:
Huang et al. (2002) Lysophospholipid mediators of immunity and neoplasia. Biochim Biophys Acta 1582:161-167.
An et al. (1998) Characterization of a novel subtype of human G proteincoupled receptor for lysophosphatidic acid. J Biol Chem 273:7906-7910.