MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT GPR85 RECEPTOR
1 vial (2 x 106) frozen cells
90%FBS, 10% DMSO
Expression vector containing full-length human GPR85 cDNA (GenBank Accession Number NM_018970.3) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM/F12, 10% FBS, 10 µg/mL puromycin
Background: GPR85 (G protein-coupled receptor 85) is also known as SREB2 (Super Conserved Receptor Expressed in Brain 2) and belongs to the family of class A (rhodopsin-like) orphan G protein-coupled receptors. The receptor is reported to be highly expressed in the brain and testis. Research has linked GPR85 to influences of brain size, behavior, and vulnerability to schizophrenia.
Application: Functional assays
Figure 1. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Yanai et al. (2016) Identification and molecular docking studies for novel inverse agonists of SREB, super conserved receptor expressed in brain. Genes to Cells 21:717-727.