HUMAN RECOMBINANT GPR52 RECEPTOR
MULTISCREEN™ β-ARRESTIN2 DIVISION ARRESTED CELL
1 vial (4 x 106) frozen cells
Cellbanker 2 (Amsbio)
Expression vector containing full-length human GPR52 cDNA (GenBank accession number NM_005684) with FLAG tag sequence at N-terminus and ARRB2 cDNA (GenBank Accession Number NM_004313.3)
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
Background: GPR52 is a class-A orphan G-protein-coupled receptor that can be found in human and mice, and is highly expressed in the brain and represents a promising therapeutic target for the treatment of Huntington’s disease and several psychiatric disorders. Once activated by antipsychotics reserpine, an increase of intracellular cAMP and its internalization is resulted. Members of this protein family contain transmembrane domain and may play a role in locomotor activity through modulation of dopamine, NMDA and ADORA2A-induced locomotor activity. They represent a promising therapeutic target for the treatment of Huntington’s disease and several psychiatric disorders.
Application: Functional assays
Figure 1. Dose-dependent stimulation from arrestin recruitment upon treatment with ligand, measured with MULTISCREENTM β-Arrestin Assay Kit (Multispan MSBA01).
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