Product Information
Catalog Number:
DC1104
Lot Number:
DC1104-020924
Quantity:
1 vial (4 x 106) frozen cells
Freeze Medium:
Cellbanker2
Host cell:
HEK293T
Transfection:
Expression vector containing full-length human FFAR2 cDNA (GenBank Accession Number NM_005306) with FLAG tag sequence at N-terminus
Recommended Storage:
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
Data Sheet
Background: GPR43, or free fatty acid receptor 2 (FFAR2), encodes a deduced 330- amino acid protein with 7 transmembrane domains. GPR43 is expressed by enteroendocrine L cells containing peptide YY in the human large intestine. The receptor binding of short- chain fatty acids potentially provides a molecular link between diet, gastrointestinal bacterial metabolism, and immune and inflammatory responses.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MULTISCREENTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
References:
Sawzdargo et al. (1997) Cluster of four novel human G protein-coupled receptor genes occurring in close proximity to CD22 gene on chromosome 19q13.1. Biochem Biophys Res Commun 239:543-547.