MOUSE RECOMBINANT GPBAR1 RECEPTOR

MULTISCREEN™ DIVISION ARRESTED CELL LINE

Product Information

Catalog Number:
DCm1361-1

Lot Number:
06/28/11

Quantity:
1 vial (4 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
CHO-K1

Transfection:
Expression vector containing full-length mouse GPBAR1 cDNA (GenBank Accession Number BC116914) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium:
DMEM/F12, 10% FBS

Stability:
Stable for 1-2 days after thawing

Data Sheet

Background: The G protein-coupled bile acid receptor GPBAR1 (or GPR131) mediates bile acids-induced rapid elevation of intracellular cAMP levels. It is implicated in the suppression of macrophage functions and regulation of energy homeostasis by bile acids.

Application: Functional assays

Figure 1. Dose-dependent increase of intracellular cAMP upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01).

References:

Maruyama et al. (2002) Identification of membrane-type receptor for bile acids (M-BAR). Biochem Biophys Res Commun 298:714-719.

Katsuma et al. (2005) Bile acids promote glucagon-like peptide-1secretion through TGR5 in a murine enteroendocrine cell line STC-1. Biochem Biophys Res Commun 329:386-390.

FOR RESEARCH USE ONLY.
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