DOG RECOMBINANT GLP-1 RECEPTOR

MULTISCREEN™ STABLE CELL LINES

Product Information

Catalog Number:
Cd1267-1

Lot Number:
Cd1267-1-022019

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Cellbanker 2 (Amsbio 11891)

Host cell:
CHO-K1

Transfection:
Expression vector containing full-length Dog GLP1R cDNA (UniProt IDJ9JHK2) with FLAG tag sequence at N- terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DME/F12, 10% FBS, 10 µg/mL puromycin

Stability:
Stable for a minimum of 2 months in continuous culture

Data Sheet

Background: Glucagon-like peptide-1 receptor (GLP1R) is a G protein-coupled receptor for the endogenous insulinotropic peptide glucagon-like peptide-1 (GLP-1). GLP1R is expressed in pancreatic cells and the nervous system, and mediate the effects of GLP-1 in regulating glucose homeostasis through multiple mechanisms including direct actions on the endocrine pancreas and indirect activation of central nervous system controlling gastric emptying, satiety, and body weight. In the brain, receptor activation elicits neurotrophic actions through protection against metabolic and oxidative insults. GLP1R is also implicated in plasticity, memory, and learning. Thus, GLP1R is a potential therapeutic target for both cognitive-enhancing and neuroprotective agents and in the treatment of stroke and Parkinsonism.

Application: Functional assays

Figure 1. Dose-dependent stimulation of intracellular cAMP accumulation upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

During et al. (2003) Glucagon-like peptide-1 receptor is involved in learning and neuroprotection. Nature Med 9:1173-1179.

Dillon et al. (1993) Cloning and functional expression of the human glucagon-like peptide-1 (GLP-1) receptor. Endocrinology 133:1907-1910.

Stoffel et al. (1993) Human glucagon-like peptide-1 receptor gene: localization to chromosome band 6p21 by fluorescence in situ hybridization and linkage of a highly polymorphic simple tandem repeat DNA polymorphism to other markers on chromosome 6. Diabetes 42:1215-1218.

FOR RESEARCH USE ONLY.
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