Product Information
Catalog Number:
DCG1337
Lot Number:
DCG1337-092524
Quantity:
1 vial (4 x 106) frozen cells
Freeze Medium: Cellbanker 2 (Amsbio 11891)
Host cell:
HEK293T Gαqi5
Transfection: Expression vector containing full-length human DRD3 cDNA (GenBank accession number NM_000796.3 with FLAG tag sequence at N-terminus
Recommended Storage:
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
Data Sheet
Background: The human dopamine receptor DRD3 (D3) is a G protein-coupled receptor for dopamine. D3 receptor belongs to the D2-like family of dopamine receptors which are widely expressed in the central nervous system. D3 is involved in movement coordination, cognition, emotion, affects memory and the regulation of prolactin secretion by the pituitary. Alterations in D3 pathways can cause pathogenesis of neurological, neuropsychiatric and hormonal disorders, including Parkinson’s Disease, schizophrenia and hyperprolactinemia.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with Multiscreen™ Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
References:
Missale, C., Nash, S. R., Robinson, S. W., Jaber, M., & Caron, M. G. (1998). Dopamine receptors: from structure to function. Physiological reviews, 78(1), 189-225.
Taylor, M., Grundt, P., Griffin, S. A., Newman, A. H., & Luedtke, R. R. (2010). Dopamine D3 receptor selective ligands with varying intrinsic efficacies at adenylyl cyclase inhibition and mitogenic signaling pathways. Synapse, 64(3), 251-266.