HUMAN RECOMBINANT D3 RECEPTOR

MULTISCREENTM DIVISION ARRESTED CELL LINE

Product Information

Catalog Number:
DCG1337

Lot Number:
DCG1337-092524

Quantity:
1 vial (4 x 106) frozen cells

Freeze Medium: Cellbanker 2 (Amsbio 11891)

Host cell:
HEK293T Gαqi5

Transfection: Expression vector containing full-length human DRD3 cDNA (GenBank accession number NM_000796.3 with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS

Data Sheet

Background: The human dopamine receptor DRD3 (D3) is a G protein-coupled receptor for dopamine. D3 receptor belongs to the D2-like family of dopamine receptors which are widely expressed in the central nervous system. D3 is involved in movement coordination, cognition, emotion, affects memory and the regulation of prolactin secretion by the pituitary. Alterations in D3 pathways can cause pathogenesis of neurological, neuropsychiatric and hormonal disorders, including Parkinson’s Disease, schizophrenia and hyperprolactinemia.

Application: Functional assays

Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with Multiscreen™ Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).

References:

Missale, C., Nash, S. R., Robinson, S. W., Jaber, M., & Caron, M. G. (1998). Dopamine receptors: from structure to function. Physiological reviews, 78(1), 189-225.

Taylor, M., Grundt, P., Griffin, S. A., Newman, A. H., & Luedtke, R. R. (2010). Dopamine D3 receptor selective ligands with varying intrinsic efficacies at adenylyl cyclase inhibition and mitogenic signaling pathways. Synapse, 64(3), 251-266.

FOR RESEARCH USE ONLY.
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