Product Information
Catalog Number:
C1336b
Lot Number:
C1336b-102924
Quantity:
1 vial (2 x 106) frozen cells
Freeze Medium: Cellbanker 2
Host cell: HEK293T
Transfection: Expression vector containing full-length human DRD2 cDNA (GenBank accession number NM_016574.4) with FLAG tag sequence at N-terminus
Recommended Storage:
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stability: In Progress
Data Sheet
Background: The human dopamine receptor DRD2 (D2) is a G protein-coupled receptor for dopamine. It can be found on postsynaptic dopaminergic neurons that are centrally involved in reward-mediating mesocorticolimbic pathways. Signaling through dopamine D2 receptors governs physiological functions related to locomotion, hormone production, and drug abuse.
Application: Functional assays

Figure 1. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP level upon treatment with ligand, measured with MULTISCREEN™ TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
References:
Grandy et al. (1989) Cloning of the cDNA and gene for a human D2 dopamine receptor. Proc Nat Acad Sci USA 86:9762-9766.
Neville et al. (2004) Identification and characterization of ANKK1: a novel kinase gene closely linked to DRD2 on chromosome band 11q23.1. Hum Mutat 23:540-545.
Usiello et al. (2000) Distinct functions of the two isoforms of dopamine D2 receptors. Nature 408:199-203.