HUMAN RECOMBINANT CYSLT2 RECEPTOR
MULTISCREEN™ STABLE CELL LINES
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Full-length human CYSLTR2 cDNA (GenBank Accession Number NM_020377)
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 200 µg/mL hygromycin
Stable after minimum two months continuous growth
Background: Cysteinyl leukotriene receptor 2 (CysLTR2) is a receptor for cysteinyl leukotrienes. There are two receptors for cysteinyl leukotrienes, CysLTR1 and CysLTR2. CysLTRs have a clear role in pathophysiological conditions such as asthma, allergic rhinitis and other nasal allergies, and have been implicated in a number of inflammatory conditions in cardiovascular and gastrointestinal diseases.
Application: Functional assays
Figure 1. Dose-dependent calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
Takasaki et al. (2000) The molecular characterization and tissue distribution of the human cysteinyl leukotriene CysLT(2) receptor. Biochem Biophys Res Commun 274:316-322.
Capra (2004) Molecular and functional aspects of human cysteinyl leukotriene receptors. Pharmacol Res 50:1-11.