HUMAN RECOMBINANT CXCR6 RECEPTOR
MULTISCREEN™ STABLE CELL LINES
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Full-length Human CXCR6 cDNA (GenBank Accession Number NM_006564) with FLAG-tag sequence at the N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM/F12, 10% FBS, 10 µg/mL puromycin
Stable in culture for minimum of two months
Background: CXCR6 (CXC-chemokine receptor type 6) is a receptor for CXC chemokine CXCL16. CXCR6 is expressed by naive CD8 T cells, natural killer T cells, a subset of memory CD4 T cells and plasma cells. CXCR6 and its ligand play an important role in the trafficking of the cells expressing them. It is demonstrated that CXCL16+ macrophages in synovial fluid of rheumatoid arthritis patients lead to recruitment of CXCR6+ memory T cells, thereby contributing to the inflammatory cascade associated with rheumatoid arthritis pathology. CXCR6 is also used as a coreceptor by simian immunodeficiency viruses (SIVs) and by strains of HIV-2 and M-tropic HIV-1.
Application: Functional assays
Figure 1. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 2. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
Deng et al. (1997) Expression cloning of new receptors used by simian and human immunodeficiency viruses. Nature 388:296-300.
Blaak et al. (2005) CCR5, GPR15, and CXCR6 are major coreceptors of human immunodeficiency virus type 2 variants isolated from individuals with and without plasma viremia. J Virol 79:1686-1700.