Product Information
Catalog Number:
C1006-1
Lot Number:
C1006-1-062210
Quantity:
1 vial (2 x 106) frozen cells
Freeze Medium:
Sigma Freezing Medium (C-6164)
Host cell:
CHO-K1
Transfection:
Full-length Human CXCR6 cDNA (GenBank Accession Number NM_006564) with FLAG-tag sequence at the N-terminus
Recommended Storage:
Liquid nitrogen upon receiving
Propagation Medium: DMEM/F12, 10% FBS, 10 µg/mL puromycin
Stability:
Stable in culture for minimum of two months
Data Sheet
Background: CXCR6 (CXC-chemokine receptor type 6) is a receptor for CXC chemokine CXCL16. CXCR6 is expressed by naive CD8 T cells, natural killer T cells, a subset of memory CD4 T cells and plasma cells. CXCR6 and its ligand play an important role in the trafficking of the cells expressing them. It is demonstrated that CXCL16+ macrophages in synovial fluid of rheumatoid arthritis patients lead to recruitment of CXCR6+ memory T cells, thereby contributing to the inflammatory cascade associated with rheumatoid arthritis pathology. CXCR6 is also used as a coreceptor by simian immunodeficiency viruses (SIVs) and by strains of HIV-2 and M-tropic HIV-1.
Application: Functional assays
Figure 1. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 2. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
References:
Deng et al. (1997) Expression cloning of new receptors used by simian and human immunodeficiency viruses. Nature 388:296-300.
Blaak et al. (2005) CCR5, GPR15, and CXCR6 are major coreceptors of human immunodeficiency virus type 2 variants isolated from individuals with and without plasma viremia. J Virol 79:1686-1700.