HUMAN RECOMBINANT CXCR5 RECEPTOR
MULTISCREEN™ DIVISION ARRESTED CELL LINE
1 vial (4 x 106) frozen cells
Expression vector containing full-length human CXCR5 cDNA (GenBank Accession Number NM_001716.2) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
Stable for 1-2 days after thawing
Background: CXCR5 (CXC-chemokine receptor 5, or Burkitt lymphoma receptor 1) is a receptor for CXCL13 and expresses in mature B-cells, a subpopulation of T-cells and Burkitt lymphoma cells. CXCR5 exerts possibly a regulatory function in Burkitt lymphoma lymphomagenesis and/or B-cell differentiation. It is a potential candidate for cell-cell interaction and activation of mature B-lymphocytes in lymphatic tissues.
Application: Functional assays
Figure 1. Dose-dependent calcium flux upon treatment with ligand, monitored with FLIPR and measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (MSCA01). No Gαqi5 transfection.
Dobner et al. (1992) Differentiation-specific expression of a novel G protein coupled receptor from Burkitt’s lymphoma. Eur J Immunol 22:2795-2799.
Campbell et al. (2003) Chemokines in the systemic organization of immunity. Immunol Rev 195:58-71.