HUMAN RECOMBINANT CCR8 RECEPTOR
MULTISCREEN™ STABLE CELL LINES
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human CCR8 cDNA (GenBank Accession Number NM_005201.2) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin, 250 μg/mL hygromycin
Background: CCR8 (C-C chemokine receptor type 8) is a receptor for the chemokines CCL1 (I-309). The receptor regulates monocyte chemotaxis and thymic cell line apoptosis, and is an additional alternative coreceptor with CD4 for HIV-1 infection. CCR8 plays a role on cell types of relevance to respiratory diseases such as asthma, chronic obstructive pulmonary disease (COPD) and chronic bronchitis. The interference of CCR8 from its ligand represents a new approach in allergy therapy.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Simmons et al. (2000) Co-receptor use by HIV and inhibition of HIV infection by chemokine receptor ligands. Immunol Rev 177:112-126.
Garcia et al. (2005) New chemokine targets for asthma therapy. Curr Allergy Asthma Rep 5:155-160