Product Information
Catalog Number:
DC1229
Lot Number:
DC1229-100215
Quantity:
1 vial (4 x 106) frozen cells
Freeze Medium:
Sigma Freezing Medium (C-6164)
Host cell:
HEK293T
Transfection:
Expression vector containing full-length human CNR1 cDNA (GenBank Accession Number NM_016083.3) with FLAG tag sequence at N-terminus
Recommended Storage:
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
Stability:
1 – 2 days after thawing
Data Sheet
Background: Cannabinoid Receptor 1, CNR1 also known as CB1, is involved in cannabinoid induced CNS effects. It acts by inhibiting intracellular adenylate cyclase activity and could be a receptor for anandamide. CNR1 is a potential target for the development of novel therapeutic drugs in the treatment of various conditions, such as pain, feeding disorders, vascular disease, Parkinson’s disease, and other central nerve system disorders.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
References:
Mendizabal and Adler-Graschinsky (2003) Cannabinoid system as a potential target for drug development in the treatment of cardiovascular disease. Curr Vasc Pharmacol 1:301-313.
Gerard et al. (1990) Nucleotide sequence of a human cannabinoid receptor cDNA. Nucleic Acids Res 18:7142.