Product Information
Catalog Number:
DC1439a
Lot Number:
DC1439a-050126
Quantity:
1 vial (4 x 106) frozen cells
Freeze Medium: Cellbanker 2
Host cell:
HEK293T
Transfection: Expression vector containing full-length human ADRB3 cDNA (GenBank Accession Number NM_000025.1) with FLAG tag sequence at N-terminus
Recommended Storage:
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
Data Sheet
Background: The ß3-adrenoceptor is also known as the atypical beta-adrenoceptor. It was considered as a potential anti-obesity target by increasing energy expenditure and lipolysis in brown and white adipose tissues of rodents. The functional receptor has been demonstrated in a variety of other tissues including blood vessel, gastrointestinal tract, muscle, and retinal endothelial cells, and its agonist evokes relaxation in some of these tissues such as gall bladder, stomach, small intestine, prostate, colon and bladder.
Application: Functional assays
Figure 1. Dose-dependent stimulation of intracellular cAMP accumulation upon treatment with ligand, measured with MULTISCREEN™ TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01).
References:
Emorine et al. (1989) Molecular characterization of the human beta 3-adrenergic receptor. Science 245:1118-1121.
Fujimura et al. (1999) Expression and possible functional role of the beta3-adrenoceptor in human and rat detrusor muscle. J Urol. 161:680-685.