HUMAN RECOMBINANT APJ RECEPTOR
MULTISCREEN™ MEMBRANE PREPARATION
1 vial (7.7 mg/mL), 1mg or 10mg
Packaging Buffer: 20mM Gly-Gly, 1 mM MgCl2, 25mM Sucrose (pH 7.2)
Expression vector containing full-length human APLNR cDNA (GenBank Accession Number NM_005161) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving. Avoid repeated freeze-thaw.
Background: APJ (also called AGTRL1, angiotensin II receptor-like 1) is a G- protein-coupled receptor that mediates diverse physiological effects of the neuropeptide apelin. It plays a role in the central and peripheral regulation of the cardiovascular system, in water and food intake, and possibly in immune function. APJ is highly expressed in the cardiovascular system and exerts the hypotensive effect in vivo and plays a counter-regulatory role against the repressor action of angiotensin II. APJ is also found to be a coreceptor for the entry of several HIV-1 and SIV strains, and apelin blocks the entry of HIV-1 and HIV-2.
Application: Functional assays
Figure 1. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Cayabyab et al. (2000) Apelin, the natural ligand of the orphan seventransmembrane receptor APJ, inhibits human immunodeficiency virus type 1 entry. J Virol 74:11972- 11976.
Katugampola et al. (2002) Discovery of recently adopted orphan receptors for apelin, urotensin II, and ghrelin identified using novel radioligands and functional role in the human cardiovascular system. Can J Physiol Pharmacol 80:369-374.