HUMAN RECOMBINANT A2B RECEPTOR
MULTISCREEN™ DIVISION ARRESTED CELL LINE
1 vial (4 x 106) frozen cells
Expression vector containing full-length human ADORA2B cDNA (GenBank accession number NM_000676.2) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
Stable for 1-2 days after thawing
Background: A2B is a receptor for adenosine. A2B receptor is upregulated during intestinal inflammation and mediates key events such as chloride, IL-6 and fibronectin secretion in intestinal epithelial cells. A2B receptor antagonists may have important clinical value in the treatment of inflammatory diseases, such as asthma and chronic obstructive pulmonary disease (COPD), as well as inflammatory bowel disease.
Application: Functional assays
Figure 1. Dose-dependent stimulation of intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01).
Hasko et al. (2008) Adenosine receptors: therapeutic aspects for inflammatory and immune diseases. Nat Rev Drug Discov 7:759-770.
Linden et al. (1999) Characterization of human A (2B) adenosine receptors: radioligand binding, wester blotting, and coupling to G(q) in human embryonic kidney 293 cells and HMC-1 mast cells. Mol Pharmacol 56:705-713.