Product Information
Catalog Number:
MC1427B
Lot Number:
MC1427B-041922
Quantity:
1 vial (6.0 mg/mL), 10mg, 5mg or 1mg
Packaging Buffer: 20 mM Gly-Gly, 1 mM MgCl2, 25 mM Sucrose (pH 7.2)
Host cell:
HEK293T
Transfection:
Expression vector containing full-length human ADORA1 cDNA (GenBank Accession Number NM_000674) with FLAG tag sequence at N-terminus
Recommended Storage:
Store at -80°C. Avoid repeated freeze-thaw.
Data Sheet
Background: Adenosine A1 receptor (ADORA1 or RDC7) is a G protein-coupled receptor for adenosine. RT-PCR detected A1 expression in the brain, colon, jejunum, ileum and kidney. A1 functions in sleep-wake regulation, the fertilization process, and modulates coronary blood flow.
Figure 1. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
References:
Ethier and Madison (2006) Adenosine A1 receptors mediate mobilization of calcium in human bronchial smooth muscle cells. Am J Respir Cell Mol Biol 35:496-502.
Zhai, W., Chen, D., Shen, H., Chen, Z., Li, H., Yu, Z., & Chen, G. (2016). A1 adenosine receptor attenuates intracerebral hemorrhage-induced secondary brain injury in rats by activating the P38-MAPKAP2-Hsp27 pathway. Molecular Brain, 9, 66. http://doi.org/10.1186/s13041-016-0247-x