Product Information
Catalog Number:
DC1427-1
Lot Number:
DC1427-1-032316
Quantity:
1 vial (4 x 106) frozen cells
Freeze Medium:
Sigma Freezing Medium (C-6164)
Host cell:
CHO-K1
Transfection:
Expression vector containing full-length human ADORA1 cDNA (GenBank Accession Number NM_000674) with FLAG tag sequence at N-terminus
Recommended Storage:
Liquid nitrogen upon receiving
Propagation Medium: DMEM-F12, 10% FBS
Stability:
Stable for 1 – 2 days after thawing
Data Sheet
Background: Adenosine A1 receptor (ADORA1 or RDC7) is a G protein- coupled receptor for adenosine. RT-PCR detected A1 expression in the brain, colon, jejunum, ileum and kidney. A1 functions in sleep-wake regulation and the fertilization process.
Application: Functional assays
Figure 1. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP accumulation upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 2. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
References:
Ethier and Madison (2006) Adenosine A1 receptors mediate mobilization of calcium in human bronchial smooth muscle cells. Am J Respir Cell Mol Biol 35:496-502.