Product Information
Catalog Number:
DC1334
Lot Number:
DC1334-053123
Quantity:
1 vial (4 x 106) frozen cells
Freeze Medium:
Cellbanker 2 (Amsbio)
Host cell:
HEK293T
Transfection:
Expression vector containing full-length human HTR7 cDNA (GenBank accession number NM_019860.2) with FLAG tag sequence at N-terminus
Recommended Storage:
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
Data Sheet
Background: The 5-HT7 receptor is a member of the GPCR superfamily of cell surface receptors and is activated by the neurotransmitter serotonin. The 5-HT7 receptor is expressed in a variety of tissues, particularly in the brain, the gastrointestinal tract, and various blood vessels. The 5-HT7 receptor plays a role in smooth muscle relaxation within the vasculature and in the gastrointestinal tract, and is involved in thermoregulation, circadian rhythm, learning and memory, and sleep. This receptor is also involved in mood regulation, suggesting that it may be a useful target in the treatment of depression.
Application: Functional assays
Figure 1. Dose-dependent stimulation of intracellular cAMP level upon treatment with ligand, measured with MULTISCREENTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01)
References:
Bard et al. (1993) Cloning of a novel human serotonin receptor (5-HT7) positively linked to adenylate cyclase. J Biol Chem 268:23422-23426.
Lovenberg et al. (1993) A novel adenylyl cyclase-activating serotonin receptor (5- HT7) implicated in the regulation of mammalian circadian rhythms. Neuron 11:449- 458.