HUMAN RECOMBINANT 5-HT4D RECEPTOR
MULTISCREENTM DIVISION ARRESTED CELL LINE
1 vial (4 x 106) frozen cells
Expression vector containing full-length human HTR4 cDNA (GenBank accession number NM_001040172.2) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM/F-12, 10% FBS
Background: The human serotonin receptor 5-HT4 is a G protein-coupled receptor. 5-HT4 receptors are located in striatal and limbic areas known to be involved in cognition and mood. 5-HT4 ligands may prove to be therapeutic in the treatment of disorders such as cardiac arrhythmias, irritable bowel syndrome and urinary incontinence. 5-HT4 receptor is also a promising target for the treatment of depression and cognitive disorders.
Application: Functional assays
Figure 1. Dose-dependent stimulation of intracellular cAMP accumulation upon treatment with ligand, measured with Multiscreen™ TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01).
Murphy SE et al. (2021) Translating the promise of 5HT4 receptor agonists for the treatment of depression. Psychol Med. 2021 May;51(7):1111-1120.
Talley (2003) Evaluation of drug treatment in irritable bowel syndrome. Br J Clin Pharmacol 56:362-369.
Van den Wyngaert et al. (1997) Cloning and expression of a human serotonin 5-HT4 receptor DNA. J Neurochem 69:1810-1819.