Product Information
Catalog Number:
CG1330
Lot Number:
CG1330-022522
Quantity:
1 vial (2 x 106) frozen cells
Freeze Medium:
Cellbanker 2 (Amsbio)
Host cell:
HEK293T Gqi5
Transfection:
Expression vector containing full-length human HTR4 cDNA (GenBank accession number NM_000870.4) with FLAG tag sequence at N-terminus
Propagation Medium: DMEM, 10% FBS, 10 μg/mL puromycin, 50 μg/mL hygromycin
Recommended Storage:
Liquid nitrogen upon receiving
Stability:
Stable for a minimum of 2 months in continuous culture
Data Sheet
Background: The human serotonin receptor 5-HT4 is a G protein-coupled receptor. 5-HT4 receptors can be found in the central nervous system and in peripheral tissues such as the alimentary tract, urinary bladder, heart and adrenal gland. 5-HT4 ligands may prove to be therapeutic in the treatment of disorders such as cardiac arrhythmias, irritable bowel syndrome and urinary incontinence.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MULTISCREEN™ Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Black line: parental cells; gray line: receptor-expressing cells.
References:
Talley (2003) Evaluation of drug treatment in irritable bowel syndrome. Br J Clin Pharmacol 56:362-369.
Van den Wyngaert et al. (1997) Cloning and expression of a human serotonin 5-HT4 receptor DNA. J Neurochem 69:1810-1819.