HUMAN RECOMBINANT 5-HT2A RECEPTOR

MULTISCREEN™ MEMBRANE PREPARATION

Product Information

Catalog Number:
MC1324-1 

Lot Number:
MC1324-1-030222 

Quantity:
6.3mg/mL (10mg, 1mg) 

Packaging Buffer: 20 mM Gly-Gly, 1 mM MgCl2, 25 mM Sucrose (pH 7.2) 

Host cell:
CHO-K1 

Transfection:
Full-length Human HTR2A cDNA (GenBank Accession Number NM_000621) with FLAG-tag sequence at the N-terminus 

Recommended Storage:
Liquid nitrogen upon receiving; avoid freeze-thaw

Data Sheet

Background: 5-HT2A (5-hydroxytryptamine receptor 2A) is a receptor for serotonin. It is expressed throughout the central nervous system in the neocortex and olfactory tubercle. Additionally, it is expressed in platelets, fibroblasts and neurons of the peripheral nervous system. 5-HT2A receptor agonists may have important clinical value in the treatment of various disorders, such as depression, anxiety, bipolar disorder and schizophrenia. It is also a receptor for the human polyomavirus, JC virus. 

Figure 1. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Black line: parental cells; gray line: receptor-expressing cells. 

References:

Meltzer and Li (2003) Serotonin receptors: their key role in drugs to treat schizophrenia. Prog Neuropsychopharmacol Biol Psychiatry 27:1159-1172. 

Porter et al. (1999) Functional characterization of agonists at recombinant human 5-HT2A, 5-HT2B, and 5-HT2C receptors in CHO-K1 cells. Br J Pharmacol 128:13-20. 

Stam et al. (1992) Genomic organization, coding sequence and functional expression of human 5-HT2 and HT1A receptor genes. Eur J Pharmacol 227:158-162. 

FOR RESEARCH USE ONLY.
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