Product Information
Catalog Number:
CA1324BA2
Lot Number:
CA1324BA2-100323
Quantity:
1 vial (2 x 106) frozen cells
Freeze Medium:
CellBanker2 (Amsbio 11891)
Host cell:
HEK293T β-Arrestin2
Transfection:
Full-length Human HTR2A cDNA (GenBank Accession Number NM_000621) with FLAG-tag sequence at the N-terminus
Propagation Medium: DMEM, 10% FBS, 1 µg/mL puromycin, 50 µg/mL hygromycin
Recommended Storage:
Liquid nitrogen upon receiving
Stability:
In progress
Data Sheet
Background: 5-HT2A (5-hydroxytryptamine receptor 2A) is a receptor for serotonin. It is expressed throughout the central nervous system in the neocortex and olfactory tubercle. Additionally, it is expressed in platelets, fibroblasts and neurons of the peripheral nervous system. 5-HT2A receptor agonists may have important clinical value in the treatment of various disorders, such as depression, anxiety, bipolar disorder and schizophrenia. It is also a receptor for the human polyomavirus, JC virus.
Application: Functional assays
Figure 1. Dose-dependent stimulation from arrestin recruitment upon treatment with ligand, measured with MULTISCREEN™ β-Arrestin Assay Kit (Multispan MSBA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
References:
Meltzer and Li (2003) Serotonin receptors: their key role in drugs to treat schizophrenia. Prog Neuropsychopharmacol Biol Psychiatry 27:1159-1172.
Porter et al. (1999) Functional characterization of agonists at recombinant human 5-HT2A, 5-HT2B, and 5-HT2C receptors in CHO-K1 cells. Br J Pharmacol 128:13-20.
Stam et al. (1992) Genomic organization, coding sequence and functional expression of human 5-HT2 and HT1A receptor genes. Eur J Pharmacol 227:158-162.