Product Information
Catalog Number:
CG1319
Lot Number:
CG1319-021622
Quantity:
1 vial (2 x 106) frozen cells
Freeze Medium:
Cellbanker 2 (Amsbio)
Host cell:
HEK293T Gqi5
Transfection:
Expression vector containing full-length human HTR1A cDNA (GenBank accession number NM_000524.2) with FLAG tag sequence at N-terminus
Propagation Medium: DMEM, 10% FBS, 10 μg/mL puromycin, 50 μg/mL hygromycin
Recommended Storage:
Liquid nitrogen upon receiving
Stability:
Stable for a minimum of 2 months in continuous culture
Data Sheet
Background: The human serotonin receptor 5-HT1A is a G protein-coupled receptor. 5-HT1A receptors can be found mainly in the cerebral cortex, hippocampus, septum, amygdala, and raphe nucelus, with lesser amounts in the basal ganglia and thalamus. 5- HT1A ligands may prove to be therapeutic in the treatment of various disorders such as depression, anxiety, and schizophrenia.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MULTISCREEN™ Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Black line: parental cells; gray line: receptor-expressing cells.
References:
Hamon et al. (1990) The main features of central 5-HT1 receptors. Neuropsychopharmacology 3:349-360.
Savitz et al. (2009) 5-HT(1A) receptor function in major depressive disorder. Prog Neurobiol 88:17-31.
Stam et al. (1992) Genomic organization, coding sequence and functional expression of human 5-HT2 and 5-HT1A receptor genes. Eur J Pharmacol 227:153-162.